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Treatment process

Clayo Clinic is the most innovative IVF Clinic in the Czech Republic

We never stop searching for ways that help us with your treatment. We improve the methods that Dr Hlinka has created and introduced into embryology during his almost 40-year medical career. At CLAYO CLINIC, we now offer these methods enhanced through continuous development.


Embryo creation

For successful pregnancy, optimally matured eggs and vital sperm are crucial. The unique Optim ICSI, treatment method developed by Dr Hlinka, helps precisely determine the optimal stage of egg maturation, thereby maximising the number of quality embryos suitable for transfer.

  • ICSI

    Intracytoplasmic sperm injection (into the oocyte)

    A type of fertilisation where the embryologist directly injects a selected sperm into the egg.

  • Optim ICSI

    our innovation

    A unique treatment method developed by Dr Daniel Hlinka, based on monitoring retrieved eggs (oocytes) in a laboratory setting. This is a non-invasive method, utilising polarisation microscopy, which recognises the maturity of the eggs and determines the optimal stage of ICSI fertilisation (intracytoplasmic sperm injection), thereby maximising the number of high-quality embryos suitable for transfer. The method is proposed mainly for poor/delayed responders.

  • Sperm retrieval and selection

    MESA / TESE, LAISS, MACS, Microchip, Swim-out


    Microsurgical Epididymal Sperm Aspiration / Testicular Sperm Extraction

    Microsurgical retrieval of sperm. It is used in patients with obstructive azoospermia.


    Laser-Assisted Immotile Sperm Selection

    The viability of sperm is a fundamental requirement for successful ICSI treatment. The LAISS method utilises a laser pulse directed at the tail of the sperm. If the tail starts to bend, the sperm is considered viable and suitable for fertilisation. It is a safe method recommended in MESA / TESE IVF cycles as well as in other cases of immotile sperm.


    Magnetic-Activated Cell Sorting

    The MACS method uses a magnetic field to select sperm with damaged genetic material. Healthy sperm are separated for further use, such as in ICSI fertilisation or freezing.


    Natural selection of sperm using a chip without the use of chemicals.


    Method of sperm selection in cases of severe OTA syndrome (oligo-astheno-terato zoospermia), i.e. the presence of a very small number of almost immobile sperm.


Embryo development and evaluation

In 2010, Dr Hlinka was one of the first in the world to introduce continuous monitoring of embryo development- the Time-lapse System (TLS ). This allows non-disturbed embryo culture and records key markers of embryo development which allows an objective embryo scoring. 

  • Time-lapse system

    our innovation

    Continuous monitoring and evaluation of embryo vitality

    The continuous recording of embryo development allows for a transparent assessment and presentation of their development from the day of fertilization to the day of transfer. This method helps to detect cell division abnormalities and also allows significantly fewer interventions in cultivation, leading to the selection of the maximum number of viable embryos for transfer.


  • Extended embryo cultivation

    The standard, uninterrupted period of embryo cultivation from fertilisation to transfer is 3 days. Extended cultivation to the blastocyst stage (blastocyst transfer) takes 5-6 days and, in combination with Time-lapse System and PGT examination, represents the most comprehensive system for embryo evaluation. (Note: blastocyst transfer is not suitable for all patients, especially not for "low responders" with a low number of retrieved eggs.)

  • Preimplantation Genetic Testing (PGT)

    A method used in the IVF process to genetically test embryos for their health. Embryos can be screened for a certain number or all chromosomes to check for any missing or extra ones. For example, Down syndrome can be ruled out. Furthermore, embryos can be tested for diseases that occur in the family and pose a realistic risk of inheritance to offspring.

  • Vitrification

    our innovation

    A method of rapid freezing used for cryopreservation of embryos and oocytes. This method is more expensive than the previously used slow freezing, but it ensures better survival of embryos or oocytes after thawing. Theoretically, embryos/oocytes can be cryopreserved in liquid nitrogen for an infinite period of time.


Embryo transfer and implantation

We do everything to make your treatment successful at the first attempt. However, sometimes embryo transfer fails or repeatedly fails. In these cases, we employ the ASET methodology to maximise the chances of successful embryo implantation.

  • LAZT (assisted hatching)

    Laser-assisted zona thinning

    The embryo is surrounded by a protective shell called the zona pellucida. When it starts to implant, this layer becomes thinner and eventually ruptures to allow the embryo to exit the shell and implant. By performing laser thinning of this shell, we ensure that the embryo expends less energy and can easily exit the shell and implant.

  • EmbryoGlue® / UTM Sage

    Media used during transfer to enhance better communication between the embryo and the uterine lining and facilitate embryo implantation after transfer. They have been proven to increase the success of embryo implantation.

  • ASET (Asynchronous embryo transfer)

    our innovation

    In the cases, when there is repeated implantation failure after transferring of high-quality embryos, we recommend the transfer of embryos at different stages of development (D3, D5). It can cover a wider implantation window of uterine receptivity and increases the chances of successful embryo implantation. This approach is a non-invasive alternative to ERA test.

Are you getting lost in the flood of terms and methods?
Take a look at a simple description of the treatment process.
More about the treatment process
The innovative methods applied in our clinic can also help you with conception as well.
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